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Please use this identifier to cite or link to this item: http://rudar.ruc.dk/handle/1800/2932

Title: Fundamental study of recombination and recombineering in Escherichia coli
Authors: Sun, Xiaohang
Huang, Yang
Advisor: Skovgaard, Ole
Keywords: recombination
recombineering
RecA
Red/ET
Examination Date: 8-Jan-2008
Issue Date: 8-Jan-2008
Abstract: Recombination and recombineering systems have been used in Escherichia coli to recombinant DNA sequences. With endonuclease and DNA lipase the bacterial plasmid and target DNA fragment can bind together and recombinant for a new DNA sequences. Red Proteins have been used in recombineering system to perform the function as the enzymes in recombination system, and faster and easier than the other way of recombinant new DNA sequences in E.coli. In this report we get to know the principles of recombination and recombineering, and how to perform the experiments. We also discussed the differences between these two technologies. Sooner this system will be important used in more widely areas such as molecular biology, medical biology and etc.
URI: http://hdl.handle.net/1800/2932
Subject: Thesis
Education: Molekyl√¶rbiologi / Molecular Biology - not master thesis
Appears in Collections:Projektrapporter og specialer / Projectreports and master thesis
Molekylærbiologi rapporter / Molecular Biology Projects

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