Transcriptional regulation of nrd gene and the effect of arginine/lysine treatment in E.coli

Abstract

The enzymes ribonucleotide reductases (RNRs) catalyse the reductive reaction, to convert ribonucleotides to deoxyribonucleotides. It’s essential for de novo DNA synthesis. A complex regulation system of RNRs has been reported, the relative proteins such as DnaA, Fis, NrdR and ArgP perform variously regulatory roles. According to already known evidences, DnaA, Fis and ArgP upregulate the expression of of RNR transcription, and NrdR plays the negative regulation. This report describes the regulatory functions of previously mentioned genes, and then introduces the experimental results that are the effects of arginine/lysine addition on both of the transcription of nrdAB gene and the DNA-ArgP protein binding reaction.